Serial Cloner

Serial Cloner is a Molecular Biology Tool which is a front end to help with DNA Cloning, Sequence Analysis, and Visualization.  It is available free for download and can be used across multiple Operating Systems, including Windows, Mac, and Linux. It can read and write files in several formats, making it portable across multiple users who use different Molecular Biology tools. Since version 2.0 of the software, it also handles Annotations and features. They are processed both in sequence and as a graphic map. The software comes with a high-quality graphics display and has a very user-friendly interface to the front end, which makes the interface very intuitive. 

Features:

• All the tools required to analyze the sequence and modify if any changes needed are available in a single window making it very easy to use.
• The fully graphical map makes it easy to extract and select a fragment or even show single, double, or multiple cutters in a single window. 
• Features can be manually entered or imported. They can be automatically found by using the scan feature.
• Sequence features are visible on the sequence map.
• There is a web browser tool within the software to allow direct import of NCBI and EMBL.
• We can use either the graphics window or the text-based site usage windows, which makes it very easy to quickly extract a sub-sequence in a selection or in any restriction sites. Also, to create new PCR based fragments or synthetic adaptors.
• We can also ligate fragments using the ligate button
• shRNA constructions can be automated
• Fragments obtained by PCR, shRNA synthesis, can be easily assembled by selecting between the restriction sites.  

In conclusion, Serial Cloner is a very user-friendly and powerful Molecular biology tool. It combines the major DNA Cloning, sequence analysis, and visualization into a single effective tool

It can read and write files in several formats, making it portable across multi-users